Microwave irradiation for rapid epidermis-dermis separation and improved epidermal cell immunodetection
We explored the effects of microwave irradiation on epidermal-dermal separation and subsequent immunostaining of epidermal cells. Epidermal sheets were obtained after incubation in 0.02 M EDTA in PBS and microwave irradiation with 4 pulses of 420 watts for 5 sec, with a total incubation period of 4...
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2002
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| Online Access: | http://hdl.handle.net/10872/12326 |
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