Comparative ultrastructure and immunolabeling of MHC-II antigens of alveolar macrophages obtained from patients with paracoccidioidomycosis and other lung diseases
Samples of alveolar macrophages (AM) obtained by bronchoalveolar lavage from patients with either paracoccidioidomycosis, silicosis, sarcoidosis, or allergic alveolitis were investigated by electron microscopy and immunocytochemistry to compare cellular ultrastructure and expression of MHC-II antige...
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1995
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| Accesso online: | http://hdl.handle.net/10872/12327 |
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| _version_ | 1870621996372459520 |
|---|---|
| bitstream.checksum.fl_str_mv | 0557d3f8559ca779355a27909a6f32d6 7c020282d630887ba819753d2e4a4c58 |
| bitstream.checksumAlgorithm.fl_str_mv | MD5 MD5 |
| bitstream.url.fl_str_mv | https://saber.ucv.ve/jspui/bitstream/10872/12327/1/Comparative%20ultrastructure%20and%20immunolabeling.pdf https://saber.ucv.ve/jspui/bitstream/10872/12327/2/license.txt |
| dc.contributor.author.none.fl_str_mv | Breta, Antonio Goihman-Yahr, Mauricio Tapia, Félix J. Istúriz, Guillermo Viloria, Nora Carrasquero, Mercedes Saavedra, Nelly Albornoz, MarIa C. Pereira, José Román, Ana Gómez, Maria H. |
| dc.creator.fl_str_mv | Breta, Antonio Goihman-Yahr, Mauricio Tapia, Félix J. Istúriz, Guillermo Viloria, Nora Carrasquero, Mercedes Saavedra, Nelly Albornoz, MarIa C. Pereira, José Román, Ana Gómez, Maria H. |
| dc.date.accessioned.none.fl_str_mv | 2015-10-16T23:34:47Z |
| dc.date.available.none.fl_str_mv | 2015-10-16T23:34:47Z |
| dc.date.issued.none.fl_str_mv | 1995-02 |
| dc.identifier.uri.none.fl_str_mv | http://hdl.handle.net/10872/12327 |
| dc.language.iso.es_VE.fl_str_mv | en |
| dc.publisher.es_VE.fl_str_mv | JOURNAL OF LEUKOCYTE BIOLOGY |
| dc.relation.ispartofseries.none.fl_str_mv | Vol. 57; |
| dc.subject.es_VE.fl_str_mv | alveolitis bronchoalveolar lavage electron microscopy sarcoidosis solicosis |
| dc.title.es_VE.fl_str_mv | Comparative ultrastructure and immunolabeling of MHC-II antigens of alveolar macrophages obtained from patients with paracoccidioidomycosis and other lung diseases |
| dc.type.es_VE.fl_str_mv | Article |
| description | Samples of alveolar macrophages (AM) obtained by bronchoalveolar lavage from patients with either paracoccidioidomycosis, silicosis, sarcoidosis, or allergic alveolitis were investigated by electron microscopy and immunocytochemistry to compare cellular ultrastructure and expression of MHC-II antigens in the AM cell surface. All samples of AM obtained from patients with these pathologies showed heterogeneous structural features. Although, this morphological diversity is also present in AM of healthy donors, our observations seem to indicate that in the diseases studied this morphofunctional diversity is associated with additional ultrastructural characteristics inherent to each disease. In paracoccidioidomycosis the proportion of vacuolated macrophages is significantly lower than in other diseases; this might indicate that in paracoccidioidomycosis the proportion of activated AM is smaller. We observed significant differences in the expression of MHC-II antigens. Silicosis, sarcoidosis, and allergic alveolitis do not differ significantly in the quantity of immunolabeled AM or in the distribution of the label. The percentage of AM from paracoccidioidomycosis that exhibit the MHC-II molecule is very low with poor immunolabeling. In this disease the low expression of the MHC-II molecule could be related to a decrease of the antigen presenting function by AM. |
| id | SABERUCV_9eebdfa7eef2cb9fcb171a5289fb9fe5 |
| language_invalid_str_mv | en |
| network_acronym_str | SABERUCV |
| network_name_str | Repositorio Institucional de la Universidad Central de Venezuela |
| oai_identifier_str | oai:saber.ucv.ve:10872/12327 |
| publishDate | 1995 |
| publishDateSort | 1995 |
| repository.mail.fl_str_mv | sfwrussians@gmail.com |
| repository.name.fl_str_mv | Saber UCV |
| repository_id_str | |
| spelling | Breta, AntonioGoihman-Yahr, MauricioTapia, Félix J.Istúriz, GuillermoViloria, NoraCarrasquero, MercedesSaavedra, NellyAlbornoz, MarIa C.Pereira, JoséRomán, AnaGómez, Maria H.2015-10-16T23:34:47Z2015-10-16T23:34:47Z1995-02http://hdl.handle.net/10872/12327Samples of alveolar macrophages (AM) obtained by bronchoalveolar lavage from patients with either paracoccidioidomycosis, silicosis, sarcoidosis, or allergic alveolitis were investigated by electron microscopy and immunocytochemistry to compare cellular ultrastructure and expression of MHC-II antigens in the AM cell surface. All samples of AM obtained from patients with these pathologies showed heterogeneous structural features. Although, this morphological diversity is also present in AM of healthy donors, our observations seem to indicate that in the diseases studied this morphofunctional diversity is associated with additional ultrastructural characteristics inherent to each disease. In paracoccidioidomycosis the proportion of vacuolated macrophages is significantly lower than in other diseases; this might indicate that in paracoccidioidomycosis the proportion of activated AM is smaller. We observed significant differences in the expression of MHC-II antigens. Silicosis, sarcoidosis, and allergic alveolitis do not differ significantly in the quantity of immunolabeled AM or in the distribution of the label. The percentage of AM from paracoccidioidomycosis that exhibit the MHC-II molecule is very low with poor immunolabeling. In this disease the low expression of the MHC-II molecule could be related to a decrease of the antigen presenting function by AM.enJOURNAL OF LEUKOCYTE BIOLOGYVol. 57;alveolitisbronchoalveolar lavageelectron microscopysarcoidosissolicosisComparative ultrastructure and immunolabeling of MHC-II antigens of alveolar macrophages obtained from patients with paracoccidioidomycosis and other lung diseasesArticleORIGINALComparative ultrastructure and immunolabeling.pdfComparative ultrastructure and immunolabeling.pdfapplication/pdf2215832https://saber.ucv.ve/jspui/bitstream/10872/12327/1/Comparative%20ultrastructure%20and%20immunolabeling.pdf0557d3f8559ca779355a27909a6f32d6MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81509https://saber.ucv.ve/jspui/bitstream/10872/12327/2/license.txt7c020282d630887ba819753d2e4a4c58MD5210872/12327oai:saber.ucv.ve:10872/123272015-10-16 23:34:47.518Saber UCVsfwrussians@gmail.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 |
| spellingShingle | Comparative ultrastructure and immunolabeling of MHC-II antigens of alveolar macrophages obtained from patients with paracoccidioidomycosis and other lung diseases alveolitis bronchoalveolar lavage electron microscopy sarcoidosis solicosis |
| title | Comparative ultrastructure and immunolabeling of MHC-II antigens of alveolar macrophages obtained from patients with paracoccidioidomycosis and other lung diseases |
| title_full | Comparative ultrastructure and immunolabeling of MHC-II antigens of alveolar macrophages obtained from patients with paracoccidioidomycosis and other lung diseases |
| title_fullStr | Comparative ultrastructure and immunolabeling of MHC-II antigens of alveolar macrophages obtained from patients with paracoccidioidomycosis and other lung diseases |
| title_full_unstemmed | Comparative ultrastructure and immunolabeling of MHC-II antigens of alveolar macrophages obtained from patients with paracoccidioidomycosis and other lung diseases |
| title_short | Comparative ultrastructure and immunolabeling of MHC-II antigens of alveolar macrophages obtained from patients with paracoccidioidomycosis and other lung diseases |
| title_sort | Comparative ultrastructure and immunolabeling of MHC-II antigens of alveolar macrophages obtained from patients with paracoccidioidomycosis and other lung diseases |
| topic | alveolitis bronchoalveolar lavage electron microscopy sarcoidosis solicosis |
| url | http://hdl.handle.net/10872/12327 |