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HCV RNA SEQUENCES IN EOSINOPHILS OF CHRONIC HCV-INFECTED PATIENTS

In the present study we examined the presence of Hepatitis C virus (HCV) RNA sequences in eosinophils (Eos) isolated from 10 chronically HCV-infected patients. At the time of the study patients showed levels of alanine aminotransferase (ALT) and aspartate aminotranferase (AST) above the normal upper...

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Published: 1999
Subjects:
Eosinophils
flow cytornetry
hepatitis C virus
infection
mononuclear cells
neutrophils
CHRONIC PATIENTS
polymorphonuclear cells
immunomagnetic absorbtion
Highly purified eosinophils
Online Access:http://hdl.handle.net/10872/14918
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bitstream.url.fl_str_mv https://saber.ucv.ve/jspui/bitstream/10872/14918/1/Documento%2087.pdf
https://saber.ucv.ve/jspui/bitstream/10872/14918/2/license.txt
https://saber.ucv.ve/jspui/bitstream/10872/14918/3/Documento%2087.pdf.txt
dc.contributor.author.none.fl_str_mv Toro, Felix
Conesa, Angela
García, Alexis H.
Deibis, Leopoldo
Bianco Colmenares, Nicolás E.
De Sanctis, Juan B.
dc.creator.fl_str_mv Toro, Felix
Conesa, Angela
García, Alexis H.
Deibis, Leopoldo
Bianco Colmenares, Nicolás E.
De Sanctis, Juan B.
dc.date.accessioned.none.fl_str_mv 2017-03-07T15:34:18Z
dc.date.available.none.fl_str_mv 2017-03-07T15:34:18Z
dc.date.issued.none.fl_str_mv 1999
dc.identifier.issn.none.fl_str_mv 0025-7850
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10872/14918
dc.language.iso.en_US.fl_str_mv en
dc.publisher.en_US.fl_str_mv JOURNAL OF MEDICINE
dc.relation.ispartofseries.none.fl_str_mv VOL. 30;Nos. 3&4 pp. 279-288
dc.subject.en_US.fl_str_mv Eosinophils
flow cytornetry
hepatitis C virus
infection
mononuclear cells
neutrophils
CHRONIC PATIENTS
polymorphonuclear cells
immunomagnetic absorbtion
Highly purified eosinophils
dc.title.en_US.fl_str_mv HCV RNA SEQUENCES IN EOSINOPHILS OF CHRONIC HCV-INFECTED PATIENTS
dc.type.en_US.fl_str_mv Article
description In the present study we examined the presence of Hepatitis C virus (HCV) RNA sequences in eosinophils (Eos) isolated from 10 chronically HCV-infected patients. At the time of the study patients showed levels of alanine aminotransferase (ALT) and aspartate aminotranferase (AST) above the normal upper limits (129 IU/L +/- 77 and 56.2 IU/L +/- 40, respectively). Absolute and relative total leukocyte and Eos counts were within the normal range. Highly purified eosinophils (> 98 %) were obtained by Ficoll-Hypaque (d= 1.114g/mL) and Percoll gradient centrifugation following by immunomagnetic absorbtion to cell specific antibodies. Mononuclear cells (MN) and neutrophils (Neu) were also purified from these patients. PCR analysis of these cell populations revealed the presence HCV RNA sequences in 4/10 Eos, 6/10 MN and 2/10 Neu cell samples. The results suggest that, in addition to MN and Neo cells, Eos might also be susceptible to HCV infection.
id SABERUCV_e0fa8270bf84178a2fd2ceffcc2cc9de
identifier_str_mv 0025-7850
language_invalid_str_mv en
network_acronym_str SABERUCV
network_name_str Repositorio Institucional de la Universidad Central de Venezuela
oai_identifier_str oai:saber.ucv.ve:10872/14918
publishDate 1999
publishDateSort 1999
repository.mail.fl_str_mv sfwrussians@gmail.com
repository.name.fl_str_mv Saber UCV
repository_id_str
spelling Toro, FelixConesa, AngelaGarcía, Alexis H.Deibis, LeopoldoBianco Colmenares, Nicolás E.De Sanctis, Juan B.2017-03-07T15:34:18Z2017-03-07T15:34:18Z19990025-7850http://hdl.handle.net/10872/14918In the present study we examined the presence of Hepatitis C virus (HCV) RNA sequences in eosinophils (Eos) isolated from 10 chronically HCV-infected patients. At the time of the study patients showed levels of alanine aminotransferase (ALT) and aspartate aminotranferase (AST) above the normal upper limits (129 IU/L +/- 77 and 56.2 IU/L +/- 40, respectively). Absolute and relative total leukocyte and Eos counts were within the normal range. Highly purified eosinophils (> 98 %) were obtained by Ficoll-Hypaque (d= 1.114g/mL) and Percoll gradient centrifugation following by immunomagnetic absorbtion to cell specific antibodies. Mononuclear cells (MN) and neutrophils (Neu) were also purified from these patients. PCR analysis of these cell populations revealed the presence HCV RNA sequences in 4/10 Eos, 6/10 MN and 2/10 Neu cell samples. The results suggest that, in addition to MN and Neo cells, Eos might also be susceptible to HCV infection.enJOURNAL OF MEDICINEVOL. 30;Nos. 3&4 pp. 279-288Eosinophilsflow cytornetryhepatitis C virusinfectionmononuclear cellsneutrophilsCHRONIC PATIENTSpolymorphonuclear cellsimmunomagnetic absorbtionHighly purified eosinophilsHCV RNA SEQUENCES IN EOSINOPHILS OF CHRONIC HCV-INFECTED PATIENTSArticleORIGINALDocumento 87.pdfDocumento 87.pdfapplication/pdf17172095https://saber.ucv.ve/jspui/bitstream/10872/14918/1/Documento%2087.pdf2a3158b830b0cb0660a9445b96052db7MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81509https://saber.ucv.ve/jspui/bitstream/10872/14918/2/license.txt7c020282d630887ba819753d2e4a4c58MD52TEXTDocumento 87.pdf.txtDocumento 87.pdf.txtExtracted texttext/plain10https://saber.ucv.ve/jspui/bitstream/10872/14918/3/Documento%2087.pdf.txt2c6eb67c8897d916ae47524b1a844d3fMD5310872/14918oai:saber.ucv.ve:10872/149182026-03-17 03:30:41.064Saber UCVsfwrussians@gmail.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
spellingShingle HCV RNA SEQUENCES IN EOSINOPHILS OF CHRONIC HCV-INFECTED PATIENTS
Eosinophils
flow cytornetry
hepatitis C virus
infection
mononuclear cells
neutrophils
CHRONIC PATIENTS
polymorphonuclear cells
immunomagnetic absorbtion
Highly purified eosinophils
title HCV RNA SEQUENCES IN EOSINOPHILS OF CHRONIC HCV-INFECTED PATIENTS
title_full HCV RNA SEQUENCES IN EOSINOPHILS OF CHRONIC HCV-INFECTED PATIENTS
title_fullStr HCV RNA SEQUENCES IN EOSINOPHILS OF CHRONIC HCV-INFECTED PATIENTS
title_full_unstemmed HCV RNA SEQUENCES IN EOSINOPHILS OF CHRONIC HCV-INFECTED PATIENTS
title_short HCV RNA SEQUENCES IN EOSINOPHILS OF CHRONIC HCV-INFECTED PATIENTS
title_sort HCV RNA SEQUENCES IN EOSINOPHILS OF CHRONIC HCV-INFECTED PATIENTS
topic Eosinophils
flow cytornetry
hepatitis C virus
infection
mononuclear cells
neutrophils
CHRONIC PATIENTS
polymorphonuclear cells
immunomagnetic absorbtion
Highly purified eosinophils
url http://hdl.handle.net/10872/14918

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