Dissociation of Purified Erythrocyte Ca2+-ATPaseb y Hydrostatic Pressure
Subunit interactions in the Ca2+-ATPase from erythrocyte plasma membranes were investigated through a combination of fluorescence spectroscopy and highpressure techniques. Application of hydrostatic pressure in the range of 1 bar to 2.4 kbar promoted full dissociation of the ATPase, as revealed by s...
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1991
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| Online Access: | http://hdl.handle.net/10872/15645 |
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| _version_ | 1870622069996126208 |
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| bitstream.checksum.fl_str_mv | 893faa57b197db593948b234728cab72 7c020282d630887ba819753d2e4a4c58 |
| bitstream.checksumAlgorithm.fl_str_mv | MD5 MD5 |
| bitstream.url.fl_str_mv | https://saber.ucv.ve/jspui/bitstream/10872/15645/1/Coelho-Sampaio%20et%20al%20JBC.pdf https://saber.ucv.ve/jspui/bitstream/10872/15645/2/license.txt |
| dc.contributor.author.none.fl_str_mv | Coelho-Sampaio, Tatiana Ferreira, Sergio T. Benaím, Gustavo Vieyra, Adalberto |
| dc.creator.fl_str_mv | Coelho-Sampaio, Tatiana Ferreira, Sergio T. Benaím, Gustavo Vieyra, Adalberto |
| dc.date.accessioned.none.fl_str_mv | 2017-04-07T15:25:14Z |
| dc.date.available.none.fl_str_mv | 2017-04-07T15:25:14Z |
| dc.date.issued.none.fl_str_mv | 1991 |
| dc.identifier.issn.none.fl_str_mv | 1083-351X |
| dc.identifier.uri.none.fl_str_mv | http://hdl.handle.net/10872/15645 |
| dc.language.iso.en_US.fl_str_mv | en |
| dc.publisher.en_US.fl_str_mv | THE JOURNAL OF BIOLOGICAL CHEMISTRY |
| dc.relation.ispartofseries.none.fl_str_mv | Vol. 266;No. 3 pp 22266-22272 |
| dc.subject.en_US.fl_str_mv | erythrocyte plasma membranes fluorescence spectroscopy highpressure techniques intrinsic fluorescence emission fluorescence polarization atmospheric pressure |
| dc.title.en_US.fl_str_mv | Dissociation of Purified Erythrocyte Ca2+-ATPaseb y Hydrostatic Pressure |
| dc.type.en_US.fl_str_mv | Article |
| description | Subunit interactions in the Ca2+-ATPase from erythrocyte plasma membranes were investigated through a combination of fluorescence spectroscopy and highpressure techniques. Application of hydrostatic pressure in the range of 1 bar to 2.4 kbar promoted full dissociation of the ATPase, as revealed by spectral shifts of the intrinsic fluorescence emission and by changes in the fluorescence polarization of dansyl-conjugated ATPase. Pressure dissociation of the ATPase displayed a dependence on protein concentration compatible with dissociation of a dimer. Calculated from pressure-dissociation curves, the standard volume change dVo for the association of subunits was 43-50 ml/mol and KO, the dissociation constant at atmospheric pressure, was 6-9 X l0-8 M. Addition of Ca2+ tabilized the dimeric ATPase structure against pressure dissociation, whereas addition of vanadate facilitated dissociation by pressure. These results suggest that intersubunit interactions depend on the equilibrium between the two major conformational states E1 and E2 of the ATPase. Addition of calmodulin in the presence of Ca2+ had no additional effect when compared to that observed in the presence of Ca2+a lone. This finding is interpreted in terms of the mechanism of Calmodulin activation of ATPase catalysis. |
| id | SABERUCV_f1d59a17bbf77b8a0ea0ce4cc8bc28de |
| identifier_str_mv | 1083-351X |
| language_invalid_str_mv | en |
| network_acronym_str | SABERUCV |
| network_name_str | Repositorio Institucional de la Universidad Central de Venezuela |
| oai_identifier_str | oai:saber.ucv.ve:10872/15645 |
| publishDate | 1991 |
| publishDateSort | 1991 |
| repository.mail.fl_str_mv | sfwrussians@gmail.com |
| repository.name.fl_str_mv | Saber UCV |
| repository_id_str | |
| spelling | Coelho-Sampaio, TatianaFerreira, Sergio T.Benaím, GustavoVieyra, Adalberto2017-04-07T15:25:14Z2017-04-07T15:25:14Z19911083-351Xhttp://hdl.handle.net/10872/15645Subunit interactions in the Ca2+-ATPase from erythrocyte plasma membranes were investigated through a combination of fluorescence spectroscopy and highpressure techniques. Application of hydrostatic pressure in the range of 1 bar to 2.4 kbar promoted full dissociation of the ATPase, as revealed by spectral shifts of the intrinsic fluorescence emission and by changes in the fluorescence polarization of dansyl-conjugated ATPase. Pressure dissociation of the ATPase displayed a dependence on protein concentration compatible with dissociation of a dimer. Calculated from pressure-dissociation curves, the standard volume change dVo for the association of subunits was 43-50 ml/mol and KO, the dissociation constant at atmospheric pressure, was 6-9 X l0-8 M. Addition of Ca2+ tabilized the dimeric ATPase structure against pressure dissociation, whereas addition of vanadate facilitated dissociation by pressure. These results suggest that intersubunit interactions depend on the equilibrium between the two major conformational states E1 and E2 of the ATPase. Addition of calmodulin in the presence of Ca2+ had no additional effect when compared to that observed in the presence of Ca2+a lone. This finding is interpreted in terms of the mechanism of Calmodulin activation of ATPase catalysis.enTHE JOURNAL OF BIOLOGICAL CHEMISTRYVol. 266;No. 3 pp 22266-22272erythrocyte plasma membranesfluorescence spectroscopyhighpressure techniquesintrinsic fluorescence emissionfluorescence polarizationatmospheric pressureDissociation of Purified Erythrocyte Ca2+-ATPaseb y Hydrostatic PressureArticleORIGINALCoelho-Sampaio et al JBC.pdfCoelho-Sampaio et al JBC.pdfapplication/pdf830848https://saber.ucv.ve/jspui/bitstream/10872/15645/1/Coelho-Sampaio%20et%20al%20JBC.pdf893faa57b197db593948b234728cab72MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81509https://saber.ucv.ve/jspui/bitstream/10872/15645/2/license.txt7c020282d630887ba819753d2e4a4c58MD5210872/15645oai:saber.ucv.ve:10872/156452017-04-25 19:13:36.865Saber UCVsfwrussians@gmail.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 |
| spellingShingle | Dissociation of Purified Erythrocyte Ca2+-ATPaseb y Hydrostatic Pressure erythrocyte plasma membranes fluorescence spectroscopy highpressure techniques intrinsic fluorescence emission fluorescence polarization atmospheric pressure |
| title | Dissociation of Purified Erythrocyte Ca2+-ATPaseb y Hydrostatic Pressure |
| title_full | Dissociation of Purified Erythrocyte Ca2+-ATPaseb y Hydrostatic Pressure |
| title_fullStr | Dissociation of Purified Erythrocyte Ca2+-ATPaseb y Hydrostatic Pressure |
| title_full_unstemmed | Dissociation of Purified Erythrocyte Ca2+-ATPaseb y Hydrostatic Pressure |
| title_short | Dissociation of Purified Erythrocyte Ca2+-ATPaseb y Hydrostatic Pressure |
| title_sort | Dissociation of Purified Erythrocyte Ca2+-ATPaseb y Hydrostatic Pressure |
| topic | erythrocyte plasma membranes fluorescence spectroscopy highpressure techniques intrinsic fluorescence emission fluorescence polarization atmospheric pressure |
| url | http://hdl.handle.net/10872/15645 |